Table 4. Protein spots picked from protein expression and IAF fluorescence gels were excised and subjected to MS for identification.
Spot | Proteins in IAF fluorescence gels | Proteins in protein expression gels | Proteins present in both IAF & Expression gels |
1 | − | + | − |
2 | − | + | − |
3 | + | − | − |
4 | + | + | + |
5 | + | − | − |
6 | + | − | − |
7 | + | + | + |
8 | − | + | − |
9 | + | − | − |
10 | − | + | − |
11 | + | − | − |
12 | + | − | − |
13 | + | − | − |
14 | − | + | − |
15 | − | + | − |
16 | + | − | − |
17 | + | − | − |
18 | + | − | − |
19 | + | − | − |
20 | − | + | − |
21 | + | + | + |
22 | − | + | − |
23 | − | + | − |
24 | + | + | + |
25 | + | − | − |
26 | + | − | − |
27 | − | + | − |
Note that spots 4, 7, 21 and 24 showed variation in both gel analyses.