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. 2014 Jul 25;9(7):e103111. doi: 10.1371/journal.pone.0103111

Table 1. The kinetic parameters of FDHs[a].

K A (mM) K iA (mM) K B (mM) k cat (1/s) k cat/K B (1/mM·s) Inline graphic
TsFDH[b] 0.281±0.081 0.005±0.002 16.24±5.39 1.769±0.441 0.109 3.2×10−1
TsFDH[c] 0.264±0.076 0.126±0.053 9.23±3.98 0.318±0.051 0.034
CbFDH[b] 0.102±0.030 0.134±0.087 8.55±2.56 1.081±0.109 0.126 3.8×10−3
CbFDH[c] 0.512±0.186 0.014±0.010 31.28±8.05 0.015±0.005 0.0004
[a]

Kinetic parameters for the forward (formate oxidation) and reverse (CO2 reduction) reactions were calculated by fitting the initial rates to equation (1).

[b]

Formate oxidation (A: NAD+, B: sodium formate).

[c]

CO2 reduction (A: NADH, B: sodium bicarbonate).