Table 1. The kinetic parameters of FDHs[a].
K A (mM) | K iA (mM) | K B (mM) | k cat (1/s) | k cat/K B (1/mM·s) | ||
TsFDH[b] | 0.281±0.081 | 0.005±0.002 | 16.24±5.39 | 1.769±0.441 | 0.109 | 3.2×10−1 |
TsFDH[c] | 0.264±0.076 | 0.126±0.053 | 9.23±3.98 | 0.318±0.051 | 0.034 | |
CbFDH[b] | 0.102±0.030 | 0.134±0.087 | 8.55±2.56 | 1.081±0.109 | 0.126 | 3.8×10−3 |
CbFDH[c] | 0.512±0.186 | 0.014±0.010 | 31.28±8.05 | 0.015±0.005 | 0.0004 |
Kinetic parameters for the forward (formate oxidation) and reverse (CO2 reduction) reactions were calculated by fitting the initial rates to equation (1).
Formate oxidation (A: NAD+, B: sodium formate).
CO2 reduction (A: NADH, B: sodium bicarbonate).