Table 1. The differential protein spots between control and DKA-exposed treatments by MALDI-TOF-MS.
Spot# | Accession NO. | Gene product | MW(kDa) | pI | Matched Peptides | Cov | Score | E-value | Fold change (Mean ± SD) | Location chromosome |
2416 | gi|57222259 | Talin-1 | 272579 | 5.86 | 28 | 14% | 68 | 0.006 | −2.19±0.46 | 10 |
2411 | gi|45387723 | Uncharacterized protein LOC402952 | 37349 | 9.23 | 7 | 36% | 58 | 0.055 | −3.64±0.05 | 21 |
2327 | gi|41944596 | Heat shock protein 4 | 95585 | 5.10 | 17 | 24% | 63 | 0.020 | −2.34±0.71 | 14 |
6459 | gi|326666090 | Multidrug resistance-associated protein 1-like | 228609 | 8.71 | 15 | 10% | 64 | 0.014 | −3.53±0.39 | 3 |
4407 | gi|366039974 | ATP synthase subunit beta, mitochondrial | 55210 | 5.14 | 12 | 30% | 76 | 0.001 | −3.51±0.05 | 11 |
3502 | gi|82181062 | Beta-coat protein | 108047 | 5.72 | 17 | 24% | 62 | 0.023 | −3.72±0.43 | 7 |
3730 | gi|300680985 | EF-hand calcium-binding domain-containing protein 4A | 46004 | 5.82 | 18 | 39% | 63 | 0.018 | −3.15±0.56 | 25 |
1325 | gi|18859049 | Myosin light chain, phosphorylatable, fast skeletal muscle a | 18967 | 4.68 | 11 | 72% | 90 | 4.1e-05 | −2.70±0.06 | 3 |
2203 | gi|62202893 | Mcm10 protein, partial | 37666 | 5.48 | 11 | 32% | 66 | 0.009 | −3.40±0.56 | 4 |
2350 | gi|18858249 | Actin, alpha cardiac muscle 1 | 42288 | 5.23 | 12 | 43% | 59 | 0.048 | −6.95±0.22 | 17 |
7301 | gi|42560193 | Actin, cytoplasmic 1, N-terminally processed | 42068 | 5.30 | 8 | 27% | 66 | 0.009 | −4.27±0.38 | 1 |
7309 | gi|55925421 | Uncharacterized protein LOC492776 | 27126 | 5.46 | 11 | 45% | 63 | 0.019 | −2.23±0.30 | 9 |
1561 | gi|125838841 | Acid sphingomyelinase-like phosphodiesterase 3b-like | 52763 | 6.73 | 10 | 21% | 67 | 0.008 | 3.76±0.33 | 16 |
1448 | gi|353558878 | Maternal embryonic leucine zipper kinase | 77612 | 8.88 | 7 | 18% | 59 | 0.050 | 3.77±0.05 | 1 |
3319 | gi|28277651 | Actin, alpha 1, skeletal muscle | 42298 | 5.23 | 15 | 47% | 72 | 0.003 | 5.02±0.13 | 17 |
4359 | gi|313482836 | Plasma membrane calcium ATPase 3a | 130715 | 5.41 | 15 | 14% | 69 | 0.005 | 2.10±0.40 | 8 |
5339 | gi|161612176 | LOC560422 protein, partial | 34141 | 8.56 | 9 | 32% | 58 | 0.062 | 5.98±0.32 | 2 |
5346 | gi|292619409 | Hypothetical protein LOC794259 | 291570 | 6.76 | 22 | 11% | 61 | 0.031 | 5.27±0.41 | 12 |
2359 | gi|62955473 | Actin, gamma-enteric smooth muscle | 42232 | 5.29 | 12 | 34% | 74 | 0.002 | 6.85±0.09 | 1 |
0297 | gi|61806478 | Cyclin-H | 37420 | 5.77 | 13 | 40% | 71 | 0.003 | 5.72±0.36 | 5 |
02105 | gi|120538416 | LOC557463 protein, partial | 42884 | 8.73 | 10 | 36% | 73 | 0.002 | 3.79±0.61 | 13 |
3225 | gi|161611678 | Zgc:171522 protein | 22889 | 9.04 | 9 | 38% | 64 | 0.016 | 4.73±0.23 | 1 |
4228 | gi|68398755 | Megakaryocyte-associated tyrosine-protein kinase | 50794 | 8.98 | 13 | 20% | 61 | 0.033 | 2.53±0.06 | 22 |
2258 | gi|41053317 | Developmentally-regulated GTP-binding protein 1 | 40726 | 8.83 | 9 | 21% | 65 | 0.013 | 4.44±0.64 | 10 |
8411 | gi|68353786 | Hypothetical protein LOC558776 | 54015 | 9.60 | 8 | 28% | 63 | 0.019 | 2.50±0.25 | 1 |
7648 | gi|326674120 | Vacuolar protein sorting-associated protein 37B-like | 34412 | 9.62 | 6 | 20% | 62 | 0.026 | 3.18±0.42 | 15 |
Note: In “fold change” column, “+” indicates up-regulation, and “−” denotes down-regulation, respectively.