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. 2014 Jul 25;9(7):e103208. doi: 10.1371/journal.pone.0103208

Figure 2. Downregulation of tumor suppressor PTEN upon HABP1 overexpression in HepG2.

Figure 2

[A-B] Downregulation of expression of the autophagic modulator PTEN in HepR21 — Autophagic modulator and also a known tumor suppressor PTEN was observed from immunoblots to have a significantly reduced expression (∼30%) in HepR21 cells compared to HepG2 after 60 h of growth, indicating a higher tumorigenicity of HepR21. Fold changes were calculated taking GAPDH as loading control, using ImageJ and expressed as mean ± standard deviation (SD) of observations in triplicate (n = 3). Statistical analysis of significance was done by Single factor one-way ANOVA (*p<0.05). [C] Immunocytochemical analysis corroborates immunoblot findings — Decreased expression of PTEN was also detected in HepR21 cells at 60 h of growth compared to that in HepG2 from cytochemical analysis.