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. 2014 Jul 25;9(7):e103525. doi: 10.1371/journal.pone.0103525

Figure 1. Maturation of E15 derived mouse primary cortical neuronal cultures.

Figure 1

Cultures were immunostained for neuronal markers (MAP2 and NeuN), a neuronal progenitor marker (Sox2), microglial marker (CD11b), oligodendrocyte marker (O4) and astrocyte marker (GFAP). E15 cultures displayed absence of neuronal progenitor cells and glial cells, containing >99% neuronal cells. Short neurites at the early stages of development (Day 2) gave rise to an extensive network of neurites by day 8. E18 astrocyte enriched cultures were used as positive control for CD11b, O4 and GFAP staining. Scale bar represents 50 µm.