Figure 2. Inhibition of the activities of ovine COX-1 (towards arachidonic acid) and human recombinant COX-2 (towards either arachidonic acid or 2-AG) by ibuprofen and its 2′- and 3′-hydroxy metabolites.

The substrates used (10 µM) are shown in the figure, as are the concentrations (in µM) of the test compounds. “F30” refers to 30 µM flurbiprofen, used as a positive control. Values are means ± s.e.m. (unless enclosed by the symbols), n = 3, of the change in oxygen utilisation following addition of enzyme to the oxygen electrode chamber.