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. 2004 May 12;6:78–82. doi: 10.1251/bpo75

Fig. 1. Representive images of FRET HAND1 homodimer experiment.

Fig. 1

HAND1YFP and HAND1T107;S109DCFP were cotransfected into HEK293 cells growing on coverslips as described, grown for 48 hr, fixed and mounted onto slides. Images prephotobleach show relative equal intensities of YFP and CFP emission. White arrow indicates the position of the cell which has been photobleached and + shows visually the increase on CFP emission. Note that the unbleached cell maintains consistent emission for both YFP and CFP.