Figure 3. Synergistic effect of Dicer1 knockdown and 5-FU on p53-mutated HACAT cells. HACAT cells were transiently transfected with Dicer1 siRNA (50 nM) or non-target control siRNA, synchronized for 48 h, and treated with increasing doses of 5-FU (10–500 µM) or vehicle for additional 48 h after cell cycle release. Results show the percentage of Annexin-FITC/PI-positive cells by flow cytometry. The data presented are means ± SD of 3 experiments.