Figure 3. Step-wise oncogenic transformation of normal human fibroblasts stimulates Rad51 promoter activity. HCA2-T cells were transfected with wild-type pRad51-Luc and various combinations of plasmids encoding oncoproteins: SV40 Large T antigen (Large T), SV40 Small T antigen (Small T), oncogenic RasV12, or GFP (control) and Rad51 promoter activity was measured by luciferase assay. Luciferase assays were performed 72 h post-transfection. The experiments were repeated at least 3 times, and error bars show s.d. *Asterisk indicates P < 0.05.