Figure 1. Overexpression of CDC55 delays mitotic entry (A) Representative images of cells overexpressing CDC55 under the strong GAL1 promoter. Cells were grown in YEP medium containing galactose (2%) for 3 h at 24 °C. (B) Schematic model of mitotic entry in budding yeast. (C) Wild-type (WT), swe1Δ or mih1Δ cells were transformed with either empty vector or GAL1-CDC55-containing plasmid. Saturated cultures were serially (10 x) diluted and spotted on SC (synthetic complete)-URA media containing either glucose (CDC55 off) or galactose (CDC55 on). Images were taken after 3 d at 24 °C.