Figure 4. Genetic interactions between ZDS1/ZDS2 and RIM15-IGO1/IGO2 (A) Overexpression of ZDS1 rescued the cold-sensitive growth defects of rim15Δ and igo1Δ igo2Δ cells. Serial dilutions of the indicated strains were spotted on SC-URA medium at 14 °C and 24 °C. 2μm ZDS1 is a high copy number plasmid expressing ZDS1. (B) Overexpression of IGO2 rescued the cold-sensitive growth defect of rim15Δ but not zds1Δ zds2Δ cells. Serial dilutions of the indicated strains were spotted on SC-URA medium at 14 °C and 24 °C. 2μm IGO2 is a high copy number plasmid expressing IGO2.