Figure 5. Overexpression of IGO2 rescues the growth defects of cdc20–3, and tom1 cells and causes a mild SAC defects. (A) igo1Δ igo2Δ cells were not sensitive to benomyl (20 μg/ml). cdc55Δ cells were used as a control. (B) igo1Δ igo2Δ cells were not defective in the SAC. Each strain was grown in YPD medium containing 15 μg/ml of nocodazole. After 3 and 4 h, rebudded cells (indicator of the SAC defects) were counted. At least 150 cells for each strain were scored. (C) Overexpression of IGO1 or IGO2 caused mild benomyl sensitivity. Serial dilutions of the strains with the indicated genotypes were spotted on YP Galactose with or without benomyl (12.5 μg/ml) at 24 °C. (D) Overexpression of IGO1 or IGO2 caused mild SAC defects. Each strain was grown in YP Galactose medium containing 15 μg/ml nocodazole. After 4 and 6 h, rebudded cells were counted. At least 100 cells for each strain were scored. (E) Overexpression of IGO2 can weakly rescue the temperature-sensitive growth defects of cdc20–3 cells. Serial dilutions of the strains with the indicated genotypes were spotted on SC-URA at the indicated temperatures. (F) Overexpression of IGO2 can weakly rescue the temperature-sensitive growth defects of tom1Δ cells. Serial dilutions of the strains with the indicated genotypes were spotted on SC-URA at the indicated temperatures.