Figure 7. Olaparib sensitivity in gastric cancer cell lines with shRNA knockdown of ATM and p53. The ATM-proficient gastric cancer cell line STKM-2 (p53 wt) was infected with lentivirus vector encoding control shRNA (A) or shp53 (B) and incubated with 0.1–3 µM olaparib in the presence of DMSO vehicle or 7.5 µM of the ATM inhibitor KU55933. (C) ATM-knockdown STKM-2 cells were infected with lentivirus vector encoding control shRNA or shp53 and incubated with 0.1–3 µM olaparib. Survival was determined using clonogenic survival assay as described in Figure 3. Each point is in triplicate in 3 independent experiments. Error bars represent SEM.