Figure 2. Methylation of EN2 CpG island in human leukemias. (A) Scheme of hEN2 gene, showing the predicted CpG island (according to ENSEMBL, in purple) and the region analyzed by bisulfite sequencing (blue), covering 68 CpG pairs, and situated approximately 300 bp upstream of EN2 ATG start codon. (B) Methylated status of CpG pairs in the selected area (open circles, unmethylated, closed circles, methylated), as analyzed by bisulfite conversion and sequencing of individual clones belonging to the indicated sample types (n = number of different samples).