Figure 6. T-cell blast crises triggered by the absence of En2 in a CML transgenic model. (A and B) Leukemic T cells in Sca1-BCRABLp210, En2^−/− mice infiltrate hematopoietic (spleen, A) and non-hematopoietic (B) tissues. Representative hematoxylin and eosin (H&E) staining analysis of the spleen (A) and lung (B) of leukemic Sca1-BCRABLp210, En2^−/− mice and control WT mice. Spleen panels show lymphoid tumor cells disrupting the normal architecture of the tissue. In the lung, the lymphoid tumor cells are infiltrating the lamina propria and the epithelium, giving rise to lymphoepithelial lesions. Magnifications are 40×. (C) The infiltrating lymphocytes in the lung are CD8⁺ T cells. Three-fold increase in the percentage of CD8+ T cells in the lungs of Sca1-BCRABLp210, En2^−/− leukemic mice. (D) Erythematous rashes in the skin of leukemic mice.