Figure 2. Tracking of PML bodies and early endosomes in mitotic cells .(A and B) Tracking of a single PML body in HaCaT cells expressing EYFP-PML1 and mCherry-Rab5 (A) or EYFP-PML1 and mCherry-2xFYVE (B). Images were captured at intervals of 2 s (every second time point [every fourth second] is displayed). The tracked area is indicated by the white rectangle. Movements in the Y-direction are visualized while movements in the X-direction are kept constant (see also Videos S1 and 2). (C) Imaging of EYFP-PML1 and mCherry-Rab5 during entry into mitosis. The nuclear boundary is indicated by white stippled lines in the first image. Arrows indicate formation of PML body–early endosome interactions at the time points when they are first detected (see also Video S3).