Figure 1. Characterizations of Myc;LOX transgenic mice and tumor inhibition by 15-LOX2. (A) Genomic PCR genotyping of single and dTg animals using β-globin for 15-LOX2 transgene (fl26),18 c-Myc for Hi-Myc transgene,23 and β-casein as internal control. WT, wild-type; fl26, 15-LOX2fl26 Tg; Myc, Hi-Myc; dTg, Myc;LOX dTg. (B) Western blot analysis of 15-LOX2 and Myc expression in single and dTg prostate lobes harvested from 3-mo-old animals of the genotypes indicated. (C) Representative whole-mount (WM; animal number indicated in the parentheses) dorsolateral (DLP) prostate HE, and Myc and 15-LOX2 IHC images of Hi-Myc vs. dTg (Myc;LOX) animals at 3 mo. Note that Hi-Myc mouse prostate lacks 15-LOX2 staining and Myc;LOX prostate lobes lack PIN and cancer lesions prevalent in Hi-Myc mice. Original magnifications of the objective lens were indicated.