Figure 1. Predicted secondary structures of the hcnA, phlA and pltA mRNA leader sequences near the translation start site when complexed with RsmA/RsmE. Below are shown the hcnA and pltA RNA oligomers used for ITC measurements. The AUG start codon, the typical GGA motif and modified nucleotides are shown in boldface and nucleotides that are part of the Shine Dalgarno consensus sequence (AAGGAGGU) are encircled. The artificial restriction sites KpnI and SphI between which synthetic oligonucleotides or gene sequences were inserted are shown. The dot (.) indicates the absence of a nucleotide. The Δ indicates deletion of the nucleotide.