Skip to main content
. 2013 Apr 23;10(6):1030–1041. doi: 10.4161/rna.24771

Table 4. Strains and plasmids used in this study.

Strain, plasmid Relevant characteristics Source or reference
E.coli    
BL21(DE3) F-ompT hsdSB (rB-,mB-) gal dcm rne-131 (λDE3) Invitrogen
P. fluorescens    
CHA0 wild type 51
CHA89 gacA::Kmr 32
Plasmid    
pME6001 Cloning vector, pBBR1MCS derivative, Gmr 14
pME6073 pME6001 derivative carrying rsmA under Plac control, Gmr 14
pME6359 pME6032 derivative carrying rsmZ under Ptac control, Tcr 41
pME6533 pME3219 with artificial restriction sites for KpnI and SphI in hcnA leader 14
pME6628 pME6533 derivative with A8 → G mutation This study
pME6702 Ptac-phlA'-'lacZ containing 92 nucleotides of phlA, Tcr 18
pME6737 pME6702 derivative with A8 → C mutation This study
pME7609 pET28b derivative carrying rsmE, Kmr 10
pME6851 pME6001 derivative carrying rsmE under Plac control, Gmr 18
pME9512 pME6533 derivative with T13 deleted This study
pME9524 pME6533 derivative with 22 nucleotides of pltA SD (+128 to ATG), inserted between KpnI and SphI in hcnA leader This study
pME9525 pME6533 derivative with 22 nucleotides of pltA SD (+128 to ATG) containing an additional T at position 13, inserted between KpnI and SphI in hcnA leader This study
pME9536 pME6533 derivative with 26 nucleotides of phlA SD (+ 376 to ATG), inserted between KpnI and SphI in hcnA leader This study
pME9537 pME6533 derivative with 26 nucleotides of phlA SD 376 to ATG) with deletion in A101 between KpnI and SphI in hcnA leader This study
pME10001 pME6533 derivative with C9 → G mutation and T13 deleted This study
pME10002 pME6533 derivative with C9 → G and G11 → A mutations and T13 deleted This study
pME10101 pME6533 derivative with 22 nucleotides of pltA SD (+128 to ATG) with G9 → A mutation, inserted between KpnI and SphI in hcnA leader This study
pME10102 pME6533 derivative with A8 → G and T13 → C mutations This study

a Oligonucleotides used for plasmid constructions are given in Table S1. The same numbers are used for oligonucleotides and corresponding plasmids. SD, Shine-Dalgarno.