Table 4. Strains and plasmids used in this study.
| Strain, plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E.coli | ||
| BL21(DE3) | F-ompT hsdSB (rB-,mB-) gal dcm rne-131 (λDE3) | Invitrogen |
| P. fluorescens | ||
| CHA0 | wild type | 51 |
| CHA89 | gacA::Kmr | 32 |
| Plasmid | ||
| pME6001 | Cloning vector, pBBR1MCS derivative, Gmr | 14 |
| pME6073 | pME6001 derivative carrying rsmA under Plac control, Gmr | 14 |
| pME6359 | pME6032 derivative carrying rsmZ under Ptac control, Tcr | 41 |
| pME6533 | pME3219 with artificial restriction sites for KpnI and SphI in hcnA leader | 14 |
| pME6628 | pME6533 derivative with A8 → G mutation | This study |
| pME6702 | Ptac-phlA'-'lacZ containing 92 nucleotides of phlA, Tcr | 18 |
| pME6737 | pME6702 derivative with A8 → C mutation | This study |
| pME7609 | pET28b derivative carrying rsmE, Kmr | 10 |
| pME6851 | pME6001 derivative carrying rsmE under Plac control, Gmr | 18 |
| pME9512 | pME6533 derivative with T13 deleted | This study |
| pME9524 | pME6533 derivative with 22 nucleotides of pltA SD (+128 to ATG), inserted between KpnI and SphI in hcnA leader | This study |
| pME9525 | pME6533 derivative with 22 nucleotides of pltA SD (+128 to ATG) containing an additional T at position 13, inserted between KpnI and SphI in hcnA leader | This study |
| pME9536 | pME6533 derivative with 26 nucleotides of phlA SD (+ 376 to ATG), inserted between KpnI and SphI in hcnA leader | This study |
| pME9537 | pME6533 derivative with 26 nucleotides of phlA SD 376 to ATG) with deletion in A101 between KpnI and SphI in hcnA leader | This study |
| pME10001 | pME6533 derivative with C9 → G mutation and T13 deleted | This study |
| pME10002 | pME6533 derivative with C9 → G and G11 → A mutations and T13 deleted | This study |
| pME10101 | pME6533 derivative with 22 nucleotides of pltA SD (+128 to ATG) with G9 → A mutation, inserted between KpnI and SphI in hcnA leader | This study |
| pME10102 | pME6533 derivative with A8 → G and T13 → C mutations | This study |
a Oligonucleotides used for plasmid constructions are given in Table S1. The same numbers are used for oligonucleotides and corresponding plasmids. SD, Shine-Dalgarno.