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. 2013 Apr 1;10(6):943–956. doi: 10.4161/rna.24453

graphic file with name rna-10-943-g6.jpg

Figure 6. RIG-I, MDA5 and MAVS mRNA levels in knockdown STING-37 cells. Total RNA was extracted from cells previously transfected with siRIG-I, siMDA5 or siMAVS STING-37 cell line, and the levels of RIG-I, MDA5 and MAVS mRNA were quantified by one-step RT-qPCR. The data were normalized to the GAPDH mRNA levels and expressed as relative levels of mRNA compared with cells without siRNA transfection (siCTRL). The mean value in STING-37 cells mock-transfected with siRNA was set at 100% of gene expression.