Figure 6. RIG-I, MDA5 and MAVS mRNA levels in knockdown STING-37 cells. Total RNA was extracted from cells previously transfected with siRIG-I, siMDA5 or siMAVS STING-37 cell line, and the levels of RIG-I, MDA5 and MAVS mRNA were quantified by one-step RT-qPCR. The data were normalized to the GAPDH mRNA levels and expressed as relative levels of mRNA compared with cells without siRNA transfection (siCTRL). The mean value in STING-37 cells mock-transfected with siRNA was set at 100% of gene expression.