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. 2013 Apr 16;10(6):957–967. doi: 10.4161/rna.24570

Table 1. Primers used.

Primer Sequence Primer Sequence
NewA 5′-GTGGAGTCTACGCGAACTTGTCCT17–3′ CDK4R822 5′-TCCACATGTCCACAGGTGTTGC-3′
NewC 5′-GTGGAGTCTACGCGAACTTGTCC-3′ CDK4F933 5′-GATGACTGGCCTCGAGATGT-3′
NewB mixture 5′-TCAGGATTGATGGTGCCTACAGC13V-3′ (V = A,G,T) CDK4R1086 5′-AGGCAGAGATTCGCTTGTGT-3′
NewD 5′-TCAGGATTGATGGTGCCTACAGC-3′ CDK4F1096 5′-TGCAGCACTCTTATCTACATAAGGAT-3′
HPRT1F123 5′-CTTCCTCCTCCTGAGCAGTC-3′ TSPAN31F73 5′-AAGCTGTCGGGGTCCTGGAA-3′
HPRT1R683 5′-AACACTTCGTGGGGTCCTTT-3′ TSPAN31F647 5′-CTTAAGCATTCAGACGAAGC-3′
CCND1F70 5′-TAGCAGCGAGCAGCAGAGTC-3′ TSPAN31R860 5′-ACCCTAGATATTCCCTAAGG-3′
CCND1F183 5′-CCCAGCTGCCCAGGAAGAGC-3′ TSPAN31R1668 5′-CTTGGAAGAAGGGACTTTCC-3′
CCND1R981 5′-TTGACTCCAGCAGGGCTTCG-3′ MYCF125 5′-GCGCTGAGTATATAAAAGCCGGTT-3′
CCND1R1067 5′-TGTGCAAGCCAGGTCCACCT-3′ MYCR838 5′-CCACCGCCGTCGTTGTCTCC-3′
CDK4F136 5′-GTATGGGGCCGTAGGAACCG-3′ BCAS4E1F 5′-TCCTGATGCTGCTCGTGGAC-3′
CDK4F665 5′ TCTGGTGACAAGTGGTGGAA 3′ BCAS3E25R 5′-CATACACAGGGACCGAGCTT-3′
NewDCF933 5′-TCAGGATTGATGGTGCCTACAGCGATGACTGGCCTCGAGATGT-3′
NewDTF647 5′-TCAGGATTGATGGTGCCTACAGCCTTAAGCATTCAGACGAAGC-3′

Note: The number in the primer indicates the first (for forward) or the last (for reverse) nucleotide of that primer in the position of the mRNA. Thus, the range between the F and R numbers should normally be the size of the RT-PCR amplified DNA fragment in agarose gel.