Table 1. Primers used.
Primer | Sequence | Primer | Sequence |
---|---|---|---|
NewA | 5′-GTGGAGTCTACGCGAACTTGTCCT17–3′ | CDK4R822 | 5′-TCCACATGTCCACAGGTGTTGC-3′ |
NewC | 5′-GTGGAGTCTACGCGAACTTGTCC-3′ | CDK4F933 | 5′-GATGACTGGCCTCGAGATGT-3′ |
NewB mixture | 5′-TCAGGATTGATGGTGCCTACAGC13V-3′ (V = A,G,T) | CDK4R1086 | 5′-AGGCAGAGATTCGCTTGTGT-3′ |
NewD | 5′-TCAGGATTGATGGTGCCTACAGC-3′ | CDK4F1096 | 5′-TGCAGCACTCTTATCTACATAAGGAT-3′ |
HPRT1F123 | 5′-CTTCCTCCTCCTGAGCAGTC-3′ | TSPAN31F73 | 5′-AAGCTGTCGGGGTCCTGGAA-3′ |
HPRT1R683 | 5′-AACACTTCGTGGGGTCCTTT-3′ | TSPAN31F647 | 5′-CTTAAGCATTCAGACGAAGC-3′ |
CCND1F70 | 5′-TAGCAGCGAGCAGCAGAGTC-3′ | TSPAN31R860 | 5′-ACCCTAGATATTCCCTAAGG-3′ |
CCND1F183 | 5′-CCCAGCTGCCCAGGAAGAGC-3′ | TSPAN31R1668 | 5′-CTTGGAAGAAGGGACTTTCC-3′ |
CCND1R981 | 5′-TTGACTCCAGCAGGGCTTCG-3′ | MYCF125 | 5′-GCGCTGAGTATATAAAAGCCGGTT-3′ |
CCND1R1067 | 5′-TGTGCAAGCCAGGTCCACCT-3′ | MYCR838 | 5′-CCACCGCCGTCGTTGTCTCC-3′ |
CDK4F136 | 5′-GTATGGGGCCGTAGGAACCG-3′ | BCAS4E1F | 5′-TCCTGATGCTGCTCGTGGAC-3′ |
CDK4F665 | 5′ TCTGGTGACAAGTGGTGGAA 3′ | BCAS3E25R | 5′-CATACACAGGGACCGAGCTT-3′ |
NewDCF933 | 5′-TCAGGATTGATGGTGCCTACAGCGATGACTGGCCTCGAGATGT-3′ | ||
NewDTF647 | 5′-TCAGGATTGATGGTGCCTACAGCCTTAAGCATTCAGACGAAGC-3′ |
Note: The number in the primer indicates the first (for forward) or the last (for reverse) nucleotide of that primer in the position of the mRNA. Thus, the range between the F and R numbers should normally be the size of the RT-PCR amplified DNA fragment in agarose gel.