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Figure 4. Mutations in the anticodon loop of the tRNACAGSer do not affect serylation. In vitro aminoacylation assays were performed with in vitro synthesized wild-type (pUC19-WT) and the mutant tRNACGASer, A35 (pUC19-A35), A35+G37 (pUC19-A35+G37) and A35+G37+G33 (pUC19-A35+G37+G33). Aminoacylation reactions were performed using purified C. albicans SerRS overexpressed in E. coli. Data represent the mean ± SD of three independent experiments.
