Figure 1. Endogenous HBO1 complex is excluded from chromatin in mitosis. HeLa cells were processed for IF; proteins were visualized with indicated antibodies. (A) The endogenous HBO1 and JADE1 localize to the nucleus in interphase cells. (B) In mitotic cells, endogenous HBO1 and JADE1 are excluded from condensed chromatin (arrow). Identical results were obtained in 293T/17 and H1299 cells. Scale Bars: 10 µm