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. 2014 Apr 16;13(12):1918–1927. doi: 10.4161/cc.28895

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Copyright © 2014 Landes Bioscience

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graphic file with name cc-13-1918-g6.jpg — Figure 6. TRAF3 requires PTEN to modulate AKT activity and cell division. (A) Immunoblot analysis of lysate from Karpas 299 cells, 24 h after transfection with control, TRAF3, TRAF3/PTEN, or PTEN siRNAs. Lysates were probed with antibodies directed to TRAF3, AKT-p (S473), PTEN, and β-actin. (B) Flow cytometry was performed on PI-stained Karpas 299 cells transfected with the indicated siRNA duplexes to analyze the cell cycle profile. (C) Immunoblot analysis of lysates from Michel cells transfected with the indicated siRNAs. Antibodies against TRAF3, AKT-p (S473), PTEN, and β-actin were used to probe the lysates. (D) Michel cells transfected with the indicated siRNA duplexes were stained with PI and analyzed by flow cytometry to ascertain cell cycle profiles. (E) Model depicting the mechanism by which TRAF3 regulates ALCL proliferation. *P < 0.001 compared with siControl; **P < 0.001 compared with siTRAF3.