Figure 6. uPAR promotes NPC cell colony formation and motility partially independent of uPA. (A) The relative uPA and uPAR mRNA levels (normalized to ACTB) in uPAR-overexpressing Hone-1 cells after uPA knockdown. The data are compared with Hone-1/Flag ^{uPA KD NC}. (B) The colony formation assay was performed to determine the effect of uPA knockdown on the growth of uPAR overexpressing Hone-1 and control cells. *P < 0.05 relative to Hone-1/uPAR ^{uPA KD NC} cells, ^#P = 0.002 compared with Hone-1/Flag ^{uPA KD NC} cells. (C) The impact of uPA knockdown on the migration and invasion of uPAR overexpressing Hone-1 cells as determined by the Transwell assays. *P < 0.005, ^#P < 0.05 relative to parental uPA knockdown NC controls. Photomicrographs are 100× (right panel). The data are presented as the mean ± SD of triplicate replicates.