Figure 1. The PHF10 gene encodes isoforms containing either PHD fingers or PDSM motif as the C-terminal domain. (A) The subunit composition of Drosophila and mammalian PBAF complexes. The homologous subunits are grouped horizontally. The PBAF-specific subunits are shown on a gray background. (B) The predicted structure of PHF10 isoforms. The PHF10 mRNA encodes isoforms that have an evolutionary conserved SAY domain and tandem PHD domains (PHF10-P) or PDSM motif (PHF10-S) at the C terminus. The short PHF10-P and PHF10-S lack 47 N-terminal amino acids. The full-length PHF10-P isoform corresponds to the PHF10 protein annotated earlier (NP_060758.2). The regions used to raise antibodies are shown at the bottom. (C) The consensus sequence for phosphorylation-dependent SUMO modification (PDSM) at the C terminus of PHF10-S isoforms. Sequences common to PHF10-P and PHF10-S are boxed. (D) Detection of PHF10 in HEK293 cell extract by western blotting with 2 different affinity purified anti-PHF10 antibodies (ab1 and ab2). (E) Each of anti-PHF10 antibodies precipitates PHF10 bands from the HEK293 extract. (F) The shRNAi knockdown of PHF10 leads to depletion of PHF10 isoforms from the HEK293 extract.