Figure 3. PHF10 isoforms are associated with PBAF signature subunits. (A) Immunoprecipitation (IP) of PBAF from HEK293 extract with antibodies against PHF10 (ab1), BAF200, and BRD7 specific PBAF subunits and a BAF155 core SWI/SNF subunit. (B) The RNAi knockdown of PHF10 leads to a decrease in the level of specific PBAF subunits in the cells. HEK293 cells were stably transfected with the construct expressing shRNA for PHF10 RNAi or with an empty vector (control). The equal amounts of protein extracts from PHF10 RNAi or control cells were loaded onto the gel, and the western blot was developed with antibodies against PBAF subunits. Tubulin was used as a loading control. (C) Transcription levels of different PBAF subunits against the background of PHF10 RNAi and in control cells as measured by qPCR.