Extended Data Table 2. Comparison of the phenotypes of published mutants^16,35–39 with fitness calculated using CirSeq.

Protein	Substitution	Fitness (CirSeq)	Phenotype	Reference
2A	H116R	1.00	WT	16
3AB	K9E	0.82	Normal plaques	35
	I12V	1.02	Normal plaques	36
	K39E	0.91	Normal plaques	36
	W42R	0.04	No plaques	36
	V44A	0.87	Normal plaques	36
	N45D	0.94	Normal plaques	36
	I46T	1.02	Normal plaques	36
	L63P	0.65	No plaques	36
	Y77H	0.88	No plaques	36
	K81E	0.88	No plaques	36
	L82P	0.20	No plaques	36
	K107E	0.36	Small plaques	35
3C	K60I	0.70	Small plaques	37
	K60T	0.92	Small plaques	37
	A61E	0	Small plaques	37
	A61V	0.69	Small plaques	37
	A66E	0.74	Small plaques	37
	A66V	0.30	Small plaques	37
	T142I	0.01	Defective viral growth	38
	H161Y	0.07	No in vitro cleavage	38
	G163V	0.56	No in vitro cleavage	38
	A172E	0.46	Impared in vitro cleavage	38
	A172V	0.15	Defective viral growth	38
3D	V33A	0.28	Loss of infectivity	39