. 2014 Mar 20;77(15):1047–1057. doi: 10.1007/s10337-014-2657-1

Table 3.

The stationary phase silanol activity and hydrophobicity evaluated for standards by means of Galushko’s test

No.	Column	Retention factor k ^a				Silanol activity	Hydrophobicity
No.	Column	Aniline	Phenol	Benzene	Toluene	Silanol activity	Hydrophobicity
1.	Discovery C₁₈	0.262 ± 0.013	0.387 ± 0.019	1.661 ± 0.083	3.263 ± 0.163	0.029	2.46
2.	Halo C₁₈	0.223 ± 0.011	0.326 ± 0.016	1.732 ± 0.087	3.618 ± 0.181	0.051	2.67
3.	XBridge C₁₈	0.346 ± 0.017	0.508 ± 0.025	2.227 ± 0.111	4.328 ± 0.216	0.045	3.28
4.	GraceSmart RP 18	0.139 ± 0.007	0.158 ± 0.008	0.595 ± 0.030	1.349 ± 0.067	0.628	0.97
5.	Betasil C₁₈	0.434 ± 0.022	0.617 ± 0.031	2,934 ± 0.147	5.902 ± 0.295	0.112	4.42
6.	Kinetex C₁₈ 100A	0.192 ± 0.010	0.274 ± 0.014	1.195 ± 0.060	2.321 ± 0.116	0.103	1.76
7.	Ib-Sil C₁₈	0.187 ± 0.009	0.223 ± 0.011	1.076 ± 0.054	2.189 ± 0.109	0.522	1.63
8.	Onyx Monolithic C₁₈	0.193 ± 0.010	0.256 ± 0.013	1.203 ± 0.060	2.357 ± 0.118	0.267	1.78
9.	Platinum C₁₈ 100A	0.246 ± 0.012	0.262 ± 0.013	1.094 ± 0.055	1.993 ± 0.100	0.810	1.54
10.	Spherisorb	0.173 ± 0.009	0.144 ± 0.007	0.345 ± 0.017	0.466 ± 0.023	1,620	0.41
11.	Spherisorb ODS2	0.465 ± 0.023	0.555 ± 0.028	2.391 ± 0.120	4.566 ± 0.228	0.517	3.48
12.	X SELECT	0.315 ± 0.016	0.397 ± 0.020	1.049 ± 0.052	1.791 ± 0.090	0.378	1.42
13.	Spherisorb ODS-2	0.340 ± 0.017	0.374 ± 0.019	1.675 ± 0.084	3.222 ± 0.161	0.729	2.45
14.	ProntoSIL	0.394 ± 0.020	0.422 ± 0.021	1.659 ± 0.083	3.201 ± 0.160	0.803	2.43
15.	Hichrom	0.383 ± 0.019	0.385 ± 0.019	1.206 ± 0.060	2.033 ± 0.102	0.989	1.62

^a k values are presented as mean value ± SD (n = 3)