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. 2014 Mar 14;210(4):656–666. doi: 10.1093/infdis/jiu158

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© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 5. — Degranulation and Toll-like receptor 2 (TLR2) expression in human neutrophils infected with Leishmania amazonensis are dependent on leukotriene B₄. Human neutrophils were treated with Zileuton for 15 minutes prior to infection with L. amazonensis–GFP (1 neutrophil to 10 L. amazonensis). After 3 hours of infection, the supernatants were collected and evaluated for degranulation by measuring metaloprotease-9 (MMP-9) release (A) and myeloperoxidase (MPO) activity (B). Cells were harvested for flow cytometry analysis of reactive oxygen species production (C) and TLR2 (D) and TLR4 (E) expression as well as the infection rate (F). *P ≤ .05; **P ≤ .01; ***P ≤ .001.