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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Virus Genes. 2014 Apr 18;49(1):157–162. doi: 10.1007/s11262-014-1068-6

Fig. 2.

Fig. 2

Localization patterns of differently truncated M1/nls-mut and swine importin α1 proteins by BIFC (a). Relative fluorescence intensity of BIFC (b). Western blotting of differently truncated M1/nls-mut in VC vector (c). VN-IP1 plasmid was transfected into PK-15 cells together with different truncated VC-M1/nls-mut plasmids (400 ng each plasmid). Twenty-four hours post transfection, fluorescence signal was observed and measured, and cells were subjected to western blotting. VC-M1/1-160/nls-mut expressed 1–160 amino acids of M1 containing mutation on 101–105 (101-AALAA-105); VC-M1/60-252/nls-mut expressed 60–252 amino acids of M1 containing mutation on 101–105 (101-AALAA-105); VC-M1/20-160/nls-mut and VC-M1/1-140/nls-mut indicated expression of 20–160 or 1–140 amino acids of M1 with mutations on 101–105 (101-AALAA-105), respectively