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Immunofluorescence assay. PK-15 cells were transfected by 3Flag-M1/60-250/nls-mut, 3Flag-M1/1-160/nls-mut, 3Flag-M1/1-140/nls-mut, and 3Flag-M1/20-160/nls-mut, respectively (400 ng per plasmid). Twenty-four hours post transfection, IFA was conducted by a monoclonal mouse anti-flag antibody (Sigma, USA)
