Skip to main content
. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Phytomedicine. 2014 Jun 7;21(10):1189–1195. doi: 10.1016/j.phymed.2014.05.004

Fig. 2. Effect of LPNS on H2O2-induced release of lactate dehydrogenase (LDH) from cultured rat astrocytes.

Fig. 2

A: Cells were pre-incubated for 24 h in the presence of different LPNS concentrations (0 (control), 2.5, 5, 10 μg/ml) and then exposed for 4 h to 0.5 mM of H2O2 in FBS free medium. LDH release was determined and expressed as a % of control. B: LDH release from astrocyte cells pretreated with 5 μg/ml LPNS for different durations (up to 48 h) before exposure to 0.5 mM H2O2. The results are expressed as a % of LDH release from vehicle-treated cells exposed to H2O2 (control). Values are means ± SE, n =3-4 independent experiments. ANOVA followed by the Dunnett’s test. *, ** and *** indicate significant differences at the p < 0.05, p < 0.01 and p < 0.001 levels, respectively.