Fig. 3. Effect of LPNS on OGD-induced release of lactate dehydrogenase (LDH) from SHSY5Y cells.
Cells were pre-incubated for 24h in media containing 0 (control), 2.5, 5 or 10 μg/ml LPNS and then underwent 6h OGD and 24h reoxygenation with glucose as described in the Methods. LDH release to media was then determined and expressed as a % of vehicle-treated cells exposed to OGD/reoxygenation. Values are means ± SE, n =3 independent experiments. ANOVA followed by the Dunnett’s test. * and ***indicate significant differences at the p < 0.05, p < 0.001 levels, respectively.