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. Author manuscript; available in PMC: 2015 Mar 28.
Published in final edited form as: Neuroendocrinology. 2014 Mar 28;99(2):94–107. doi: 10.1159/000362280

Figure 3.

Figure 3

Expression profile of Kiss1 mRNA transcriptional variants in rat POA and MBH during female peripubertal development. (a) Schematic diagram showing locations of RT-PCR primers used for the analysis of Kiss1 mRNA expression. Boxes and lines represent exons and introns, respectively. Untranslated regions are represented by white boxes and coding regions are shown in gray. (b) The abundance of E1b-E2a and E1b-E2b mRNA transcripts increases in both rat POA and MBH during pre-and peripubertal development (c) Expression of the alternatively transcribed E1a-containing transcript does not change during female peripubertal development. The results are expressed in arbitrary units (ratios between Kiss1 mRNA and Ppia mRNA). Vertical bars represent the mean ± SEM; n = 4–9 animals per group. ***, P < 0.001 vs. EJ and LJ groups (ANOVA followed by the Student-Newman-Keuls multiple comparison test). f = forward; r = reverse.