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. Author manuscript; available in PMC: 2015 Jul 25.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Jun 26;450(2):1027–1031. doi: 10.1016/j.bbrc.2014.06.095

Fig. 2.

Fig. 2

Effect of NHE1 ablation on regulators and markers of cellular stress. RT-PCR analysis was carried out on wild-type (WT) and NHE1-null (KO) samples as described in Fig. 1. Results show that mRNA levels encoding sestrin 2 (SESN2) were elevated (A) while sestrin 3 (SESN3) was unaltered (B) in KO livers. Immunoblot analysis of total protein homogenates revealed that, when normalized to actin, levels of superoxide dismutase 1 (SOD1) (C) and α-Bcrystallin (CRYAB) (D) were reduced in KO livers when compared to WT controls. Expression of mitochondrial SOD2, cytochrome C oxidase subunit IV (COX4) and heat shock protein HSP60 was unaltered. n = at least 8 for each genotype for RT-PCR analysis; at least 4 of each genotype for immunoblot analysis. Values are mean ± SEM. *p < 0.05, KO vs WT.