Fig. 4.

Hepatic insulin signaling is better preserved in NHE1-null mice. Wild-type (WT) and NHE1-null (KO) mice were maintained on a high-fat diet (60% of kcals in fat) for 8 weeks and then treated with insulin as described above. Immunoblot analysis of total protein homogenates of livers showed that (A) when normalized to total Akt levels, phosphorylation of Akt on Ser473 (p-Akt) was higher in KO livers when compared to similarly treated WT controls. Results also showed that (B) expression of SOD1 and CRYAB, when normalized to actin, was reduced in KO livers even after exposure to a high-fat diet. n = at least 3 for each genotype. Values are mean ± SEM. *p < 0.05, KO vs WT.