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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 Apr 16;0:51–59. doi: 10.1016/j.freeradbiomed.2014.04.014

Figure 3.

Figure 3

LPS induces tyrosine nitration in BV2 cells. (A) Confocal images showing anti-3-nitro-tyrosine staining of BV2 cells treated with 500 ng/ml LPS and 50 mM DMPO for 24 hours in the presence and absence of pretreatment with FeTPPS (10 µM), L-NAME (100 µM), 1400W (10 µM), and apocynin (1 mM). (B) Anti-nitrotyrosine ELISA of BV2 cells treated with 500 ng/ml LPS and 50 mM DMPO for 24 hours in the presence and absence of pretreatment with FeTPPS (10 µM), L-NAME (100 µM), 1400W (10 µM), and apocynin (1 mM). Data show mean values ± SEM or representative images from three independent experiments (n=6). (*** P<0.001, with respect to control, and ### P<0.001, with respect to the LPS-treated group.)