Elevated binding densities at ends reflect preferential interactions. Away from the ends, uniform binding densities are incompatible with DNA sequence or base-composition preference. Because the unmodified DNA ends used in these experiments cannot be distinguished, locations are reported in units of DNA length from 0% (at a DNA end) to 50% (at the DNA center). Low and high [AGT] correspond to incubations at 2 μM (grey, 2.6 +/− 0.3 peaks per DNA, n = 220), and 12 μM AGT (white, 4.6 +/− 2.2 peaks per DNA, n = 159), respectively. For comparison, DNA in the absence of protein is shown (black, 0.7 peaks per DNA, n = 54), demonstrating a low background, likely due to salt contaminations on the DNA.