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. 2014 Mar 17;18(6):1104–1112. doi: 10.1111/jcmm.12255

Figure 3.

Figure 3

miR-155 targets and FIZZ1 are up-regulated in bone marrow–derived macrophages (BMDMs) after polarization towards M2 phenotype. (A) Quantitative analysis of specific markers for M1 polarization (iNOS, TNF-α and MCP-1) and M2 polarization (Arg-1, YM1 and FIZZ1) in unpolarized BMDM (M0) and BMDMs polarized towards the M1 and M2 phenotypes. M2 macrophages derived from miR-155−/− mice show increased FIZZ1 expression. Graphs show fold change ± SEM of representative results from four independent experiments, #P < 0.1 with respect to WT group. (B) Quantitative analysis of validated miR-155 targets show no differences in unpolarized BMDM (M0) or BMDM polarized into an M1 phenotype. M2 phenotype shows up-regulation of the validated miR-155 targets: BCL6, RhoA and SHIP1. Graphs show fold change ± SEM of representative results from four independent experiments, *P < 0.05 and #P < 0.1 with respect to WT group.