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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: DNA Repair (Amst). 2014 Feb 20;20:23–31. doi: 10.1016/j.dnarep.2014.01.007

Figure 2. Sample configuration for SM DNA tightrope experiments.

Figure 2

(A) λ-DNA is extended across 5 µm beads using hydrodynamic flow. (B) Image of Qdot labeled glycosylase bound to DNA stretched between two beads. (C) Conjugation scheme for attachment of Qdot to glycosylase. A streptavidin-modified PEG coated QD is attached through biotin to an antibody that is specific for the 6-histidine tag on the C-terminus of the glycosylase (not to scale).