Table 1.
Clones containing glycosyl hydrolase genes from metagenomic libraries constructed from different environmental samples
| Library source | Screening number (Library size) | Active clone | Enzyme name | Sequence name (accession no.) | |
|---|---|---|---|---|---|
| Soil | 30,000 (130 Mb) | n.d. | - | - | |
| Avicel 1st enrichment culture | 25,000 (108 Mb) | Cellulase | p1a1 | C1A1 | 1A1 (AB922008) |
| Cellulase | p1a2, p1a3, p1a4 | C1A2 | 1A2 (AB922009) | ||
| Avicel 2nd enrichment culture | 32,000 (138 Mb) | Cellulase | p2a1 | C2A1 | 2A1 (AB922010) |
| Cellulase | p2a2, p2a4 | C1A2 | 1A2 (AB922009) | ||
| Cellulase | p2a3 | C2A3 | 2A3 (AB922011) | ||
| Pulp 1st enrichment culture | 23,000 (100 Mb) | n.d. | - | - | |
| Pulp 2nd enrichment culture | 40,000 (172 Mb) | Cellulase | p2p3, p2p4, p2p4 | C2P3 | 2P3 (AB922013) |
| Xylanase | p2p1 | X2P1 | 2P1 (AB922012) | ||