Skip to main content
. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Bioorg Med Chem Lett. 2013 Nov 1;23(24):6695–6699. doi: 10.1016/j.bmcl.2013.10.030

Figure 2.

Figure 2

The HTG:TO-neo binding isotherms. The HTG was titrated (25–180 nM) into 100 nM TO-neo in 10 mM sodium cacodylate, 0.5 mM EDTA, 100 mM NaCl at pH 7.0 after incubating for (a) 0 h (b) 6 h (c) 12 h or in 10 mM HEPES, 100 mM KCl at pH 7.0 after incubating for (d) 0 h (e) 6 h (f) 12 h. Titrations were performed in a 96-well round bottom Greneir black plate with an excitation wavelength of 485 nm and emission fluorescence measured at 535 nm from 100 reads, and all points of the isotherms were the average of two wells. Time trials were determined from separate plates to insure minimal photo bleaching resulting from multiple measurements.