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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Methods. 2014 Apr 26;68(3):450–457. doi: 10.1016/j.ymeth.2014.04.015

Figure 1.

Figure 1

Changes in agar temperature with time. Agar plates moved from a maintenance temperature (e.g. 20°C) to a 37°C incubator do not come immediately up to the temperature of the incubator. The most dramatic increase in temperature occurs within the first 5 minutes. Time -1 = room temperature immediately before being placed into the incubator, time 0 = immediately after being placed onto the pre-warmed gel pad and covered with other warm gel pads as well as possible without disturbing the thermocouple probes. Temperatures were recorded using a thermocouple attached to a digital thermometer (Omega Engineering model HH506RA).