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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Methods. 2014 Apr 26;68(3):450–457. doi: 10.1016/j.ymeth.2014.04.015

Figure 3.

Figure 3

Incubator set up for heat shock experiments. The image below shows our efforts to get as much warm surface area as possible into the incubator. Shown is a Forma Scientific water-jacketed, stacked incubator, model 3327. The top shelf was chosen for the experimental space because, without the fan running, it was the shelf that most frequently stayed at the temperature set on the incubator. The middle shelf tended to be lower than desired and the bottom shelf lower still. Although this was less than a 1°C difference from top to bottom, that can be enough to affect the outcome of an experiment. The bottom shelf has a desktop file organizer filled with gelatinous freezer packs and a small battery-operated fan. The gel pad on the second shelf is used on top of the experimental plates during heat shock.