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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Methods. 2014 Apr 2;68(3):518–528. doi: 10.1016/j.ymeth.2014.03.028

Figure 1. Clathrin distribution during early zygotic development.

Figure 1

Individual images of GFP:CLIC-1 demonstrate its distribution at various times relative to zygote ovulation. Arrows highlight clathrin accumulation at the plasma membrane prior to ovulation (−3 min timepoint) and coincident with meiosis II (+20 min timepoint) or its absence at the plasma membrane (+10 min timepoint). Insets are 3x zoomed views of the areas highlighted by arrows. Scale bar, 20 μm. Inset scale bars, 5 μm.