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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Methods. 2014 Apr 2;68(3):518–528. doi: 10.1016/j.ymeth.2014.03.028

Figure 6. ESCRT-I dynamics during early zygotic development.

Figure 6

Individual images of GFP:MVB-12 illustrate its distribution at various times relative to zygote ovulation. Long arrows highlight GFP:MVB-12 accumulation on early endosomes near the plasma membrane during the second wave of clathrin-mediated endocytosis (+23 min timepoint). Short arrowheads highlight the localization of ESCRT-I to midbodies. Inset is a 3x zoomed view of the area highlighted by an arrow. Scale bar, 20 μm. Inset scale bar, 5 μm.