Figure 1. Spectroscopic characterization of the hCBS native state in the absence and presence of NaCl.
A) Polydispersity and hydrodynamic radius of hCBS in the presence of different salt concentrations obtained from DLS experiments. B) Far-UV circular dichroism spectra. Inset: molar ellipticity at 222 nm at different NaCl concentrations. C) Near-UV/visible absorption spectra. Spectra in the absence or presence of salt are undistinguishable. Inset: absorption intensities at 429 and 365 nm as a function of NaCl concentration. D) Near-UV/visible circular dichroism spectra. In panels B-D, the lines follows the legend of panel B. Errors in panel A are mean ± s.d. from three different measurements.