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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 26;1844(9):1453–1462. doi: 10.1016/j.bbapap.2014.04.015

Figure 2. Effect of salt on the activity and regulation of hCBS.

Figure 2

A) Effect of 200 mM NaCl on the specific activity of hCBS in the absence/presence of 300 μM SAM. The extent of SAM mediated activation of hCBS is indicated as a x-fold. B) SAM concentration dependence of hCBS activity in the absence and presence of 200 mM NaCl. SAM concentration required for half-activation are 3.7±0.2 μM (no salt) and 14.4±0.9μM (200 mM NaCl). Note the logarithmic scale of the x-axis. Experiments performed in 20 mM Hepes pH 7.4. Activity measurements were performed in 20 mM Hepes pH 7.4, 10 mM serine and 10 mM homocysteine. Error bars in panel B are S.D. from triplicate experiments.