Figure 7. Structural and energetic effects of 200 mM NaCl on the kinetic stability of RDs and CDs.

A) Changes in activation energetic parameters by salt (200 mM – 0 mM NaCl). Errors are those propagated from denaturation rate constants determined at different scan rates (activation ΔΔG), from experimentally determined E_a values at different scan rates (activation ΔΔH) and from those propagated using activation ΔΔG and ΔΔH (activation -TΔΔS). B) Kinetic (m^ǂ) and equilibrium (m_eq) m values for the RDs and CDs in the absence and presence of 200 mM NaCl. m^ǂ values are reported as mean ± s.d. from three different scan rates determined using equation 5; C) energetic and D) structural effects from unfolding and solvation components in the absence and presence of salt. Errors in panels C and D are propagated from experimental errors in m^ǂ values.